This diagnostic process in microbiology identifies microorganisms able to hydrolyzing urea. The method depends on an enzyme that breaks down urea into ammonia and carbon dioxide. The launched ammonia will increase the pH of the expansion medium. This pH shift is detectable by a coloration change in a pH indicator, reminiscent of phenol purple, which transitions from yellow to pink or magenta in alkaline situations. A constructive outcome, indicated by this coloration change, signifies the presence of a urease-producing organism.
The importance of this analytical approach lies in its means to distinguish and determine varied bacterial species. Particularly, it aids within the detection of pathogens like Proteus vulgaris, identified for its potent urease exercise, which is linked to the formation of urinary stones. Its use permits for faster identification of those particular organisms. Historic context demonstrates its essential function in scientific microbiology for many years, providing a speedy and cost-effective preliminary check for characterizing bacterial isolates.
