This diagnostic process identifies inducible clindamycin resistance in Staphylococcus aureus and different staphylococci, in addition to beta-hemolytic streptococci. A optimistic end result signifies that whereas the organism could seem prone to clindamycin in vitro, resistance could be induced by erythromycin publicity, resulting in remedy failure. This check is carried out by putting erythromycin and clindamycin disks on an agar plate inoculated with the micro organism of curiosity. After incubation, the zone of inhibition across the clindamycin disk is examined for flattening, or a “D-zone,” adjoining to the erythromycin disk.
The check is essential for guiding antibiotic choice, significantly when treating infections brought on by Staphylococcus aureus. It prevents the inappropriate use of clindamycin in instances the place resistance can emerge throughout remedy. Implementing this check helps optimize affected person outcomes by making certain that antibiotics prescribed are efficient towards the particular bacterial pressure inflicting the an infection. Traditionally, the popularity of inducible resistance patterns led to the event and widespread adoption of this methodology in medical microbiology laboratories.
